Bio-control of Acinetobacter baumannii by Bdellovibrio as a predatory bacteria

Management of antimicrobial-resistant Acinetobacter baumanni is a great challenge for clinical microbiologist. Whereas use the predatory bacteria is the best way to treat infection diseases  caused by antibiotic-resistant bacteria, the aim of this study was to use the autochthonous Bdellovibrio potential to prey Acinetobacter baumannii as a biological control agent.

To evaluate the effect of autochthonous Bdellovibrio  on  Acinetobacter baumannii, as a biological control agent, plaque perdition assay, reduction in optical density (OD) and reduction in host cells viability by colony forming unit (CFU) counting in cocultures after 0,24, 48 hours  and assay of killing efficiency were carried out.

Clear plaques were observed after 3-6 days of incubation. In co-cultures, the CFU enumeration of Acinetobacter baumannii was decreased after 48 hours. Also, after 48 hours, OD was decreased 0.7unit. In this research the efficiency ofBdellovibriokilling for Acinetobacter baumannii was 83%.

Base on the results, Bdellovibrio can prey Acinetobacter baumannii as a prey cell. Therefore utilize of  Bdellovibrio spp., as biological control agent, for treatment of antimicrobial-resistant Acinetobacter baumannii infection suggested in future study.