Antibacterial effect of mentha spicata, cuminum cyminum essential oils and bacteriocin enterocin P peptide on some bovine mastitis bacteria in vitro

Nowadays, milk and dairy products form an important part of the requirements of the people around the world. Animal diseases have important impacts on animal productivity and welfare and on human health. Mastitis is the most common disease in dairy cattle. Unfortunately, using common antibiotics to treat animal infection is increasing in which leads to undesirable side effects such as antibiotic residuals in animal products and occurrence bacterial resistance to antibiotics. Hence, the use of natural alternatives such as antimicrobial peptides and essential oils has been considered. In this study the antibacterial effects of two bacteriocin enterocin P and thanatin peptides with two essential oils of mentha spicata and cuminum cyminum against some mastitis pathogens were evaluated. The recombinant bacteriocin enterocin p peptide were expressed in CHO cells. The extraction of mentha spicata and cuminum cyminum essential oils was performed by Clevenger apparatus. Finally, the minimum inhibitory concentration and minimum bactericidal concentration were tested on bacteria. The results have shown that the two peptides have stronger antibacterial effects at lower concentrations than the two essential oils. Staphylococcus aurous and Escherichia coli were sensitive to bacteriocin enterocin p and thanatin peptide respectively. Since the benefits of using peptides over essential oils associated with their high thermal stability, resistant to oxidation and the ability of peptide modification, it is hope that antimicrobial peptides provide an excellent potential strategy for treating animal diseases in future.