Evaluation of Antimicrobial Compounds Produced ByExiguobacterium Acetylicum and Analysis of its Supernatant By GC/MS Method

Aim and

Many microorganisms produce antimicrobial agents protecting them in extreme environmental conditions. Exiguobacterium sp. has particularly been adapted to their environment and is of wide diversity depending on the species and environmental conditions. The present study aims to study on the isolation, identification, and examination of antimicrobial property of bioactive compounds produced by Exiguobacterium acetylicum PTCC 1765.

E. acetylicum was taken from Iranian Research Organization for Science and Technology and was cultured in Corynebacterium broth and nutrient broth. Agar diffusion and well diffusion techniques were used to study the antimicrobial effect of supernatant of this bacterium against pathogenic and standard strains of bacteria such as Escherichia coli, Salmonella enterica, Staphylococcus aureus, Shigella dysenteriae and compounds with antibacterial property were identified using GC/MS.

E. acetylicum culture’s supernatant displayed a good antimicrobial property. Its greatest effect was observed during Well diffusion technique over salmonella entrica servpvar paratiphi PTCC 1231 with 8.5 mm zone of inhibition based on GC/MS analysis 2- ethyl  hexyl parametoxy cynamate, 1-2-benzen-dicarboxilic acid, 1-7- dimethyl 3- phenil- thilidin byciclo (1-2-2) – heptanes-2- on and phenol 2-4 bis(1-1-dimethyle ethyle)were dominant compounds.

Nowadays, by increased resistance of antibiotics and the complexities resulted from consumption of chemical pharmaceutics, using alternative medicines, seems necessary. The supernatant of E. acetylicum culture having high antimicrobial capability and useful compounds with restricting property can be suggested as an antimicrobial agent. While comprehension studies are needed to affirm the effect of this bacteria and its resulting metabolites in the human body conditions.