Restriction Enzyme Analysis of Thymidine Kinase Gene in Four Iranian Isolates of Herpes Simplex Virus Type 1

The thymidine kinase (TK) gene of four Iranian herpes simple.\; virus-I isolates was ampli lied and the electrophoretic pallerns or the gene were compared after digestion with six restriction endonucleases including Apal, Aval. Bg/I, Drall. EcoRV and Pst!. DN8A of each virus was 1irst extracted scparately l1y Iysis butTer and phenol/chloroform method. Their TK genes WC1'<: ampli lied lIsing a pair or special primers. which were designed by Gene 8RlInner software. The polymerase chain reaction product of eaeh TK gene was digested with the restriction enzymes separately and electrophoresed in polyacrylamidc gel. The results indicatc that there was no diffcrcnce among the c1ectrophoretic pattcrns or the isolates TK gene.