Histomorphometric Changes of Ovary in Polycystic Ovary Syndrome in Rats after Administration of Evening primrose Oil

Polycystic ovary syndrome (PCOS) is a very prevalent and complex hormonal imbalance that affects almost 5 to 10% of women. Therefore, the aim of this study was to determine and histomorphometric changes of ovary in polycystic ovary syndrome induced in rats by letrozole after administration of Evening primrose oil.

In this experimental study conducted in 2019, 32 healthy rats with a mean weight of 200 to 250 g were included in the study. The mice were randomly divided into 4 equal groups; Group 1: EPO at dose of 500 mg/kg was injected intraperitoneally for twenty-eight days. Group 2: PCOS was administered orally at letrozole at dose of 1 mg/kg body weight for 28 days. The third group: PCOS was induced and for twenty-eight days, EPO at dose of 500 mg/kg was administered by intraperitoneal injection and the fourth group was induced by PCOS and EPO at a dose of 1000 mg/kg for twenty-eight days. It was administered by intraperitoneal injection. Finally, after fixing the ovarian tissues, histological sections were prepared serially and folliculogenesis was performed based on counting different types of follicles. Data were analyzed using one-way ANOVA and Tukey tests.

Histomorphometric data showed that EPO at dose of 500 mg/kg increased corpus luteum in the PCOS group treated with EPO compared with the control group (p <0.05). EPO also reduced cystic follicles in both doses compared to the control group (p <0.05)

According to the results of histomorphometric study of ovarian tissue, it can be suggested that EPO can have beneficial effects on polycystic ovary syndrome by improving the maturation of follicles.