Effects of Temperature, Propagation Media and Scale Position on Bulblet Regeneration of Chelcheragh Lily (Lilium ledebouri Boiss.) by Scaling Method

Iran is one of the most important distribution areas of Chelcheragh lily
(Lilium ledebouri) in the world. In the process of identification, evaluation and finally commercialization of potential germplasms, propagation of a new plant (new crop) is one of the most important factors that should be investigated. To investigate the influence of scale position, different temperatures and media on the regenerated bulblets indices, and also effect of cold treatment on removal of dormancy of bulblets, separate experiments were carried out. Results showed that outer and middle scales at 20 and 25 oC regenerated more bulblets with better properties than inner scales. Regeneration of bulblets were inhibited completely at 30 oC. The highest number of bulblets were regenerated in middle scales and in tree bark+ tea wastes medium that was not significantly different from those regenerated with outer scale and tree bark+tea wastes, peat+perlite, and sand+perlite. Regenerated bulblets were non-green leaf bulblets and dormant. Bulblets needed to 3-5 oC for 6-8 weeks, until 70-84% of them can produce leaf. In this study, it was also cleared that number of regenerated bulblets in cold passed bulbs treatment did not differ from those of non-cold passed bulb treatment.