Effect of Cinnamic acid on the activity of phenylalanine ammonialyase (PAL) and tyrosine ammonialyase (TAL) enzymes and some physiological characteristics of tobacco plant (Nicotiana rustica L.) under salinity stress in vitro calture

The aim of this study was to investigate the effect of Cinnamic acid (CA) on some physiological characteristics and salt tolerance of tobacco (Nicotiana rustica L.)  plants.

In this study, explants of tobacco grown in MS medium was transferred to MS media containing CA (concentrations of 0, 5, 10 and 20 mg / l) and NaCl salt (concentrations of 0, 100 and 200 mM). After four weeks of treatment, the necessary analysis contains measurement activity of phenylalanine ammonialyase (PAL) and tyrosine ammonialyase (TAL), total phenolic compound and total protein, accumulation of flavonoid, anthocyanins, total antioxidant capacity, hydrogen peroxide (H2O2), potassium and sodium and proline done on the treated plants.

The results showed that the treatment of plants with CA increased the activity of PAL and TAL, total phenolic compounds and total protein. CA treatment also increased the accumulation of flavonoids, anthocyanins and proline as well as potassium and total antioxidant capacity. But the amount of sodium and H2O2 decreased as a result of CA treatment.

It is possible that CA increases the salt tolerance of tobacco by increasing the amount of total phenolic compounds as well as proline under salt stress conditions.