The effect of adding pentoxifylline to the Ross rooster semen on quality parameters of spermatozoa during 6 hours of storage at cooling temperature

The most important factors involved in sperm fertility are motility, morphology and sperm membrane integrity. The studies show that, the addition of Pentoxifylline(PTX) can increase sperm motility by increasing energy production. The aim of the present study was to investigate the effect of adding Pentoxifylline to rooster semen on qualitative parameters of rooster sperm after 6 hours of storage at 4°C. Semen samples were collected from 15 Ross 308 roosters at 28 weeks of age. After initial evaluations, semen samples were mixed together to eliminate individual differences. After diluting the semen and adding different levels of Pentoxifylline (0, 0.25, 0.5, 0.75 and 1 μM), the samples were kept at 4°C for 6 hours. Then, sperm kinetic parameters were evaluated. The results showed that the addition of 0.75 μM PTX significantly improved progressive mobility, curvilinear velocity, viability and plasma membrane integrity of spermatozoa compared to the control group (P<0.05).  In overall, it can be concluded that methylxanthine-derived drugs such as Pentoxifylline can have a significant effect on the motility, viability and plasma membrane integrity of rooster sperm in the short period of time.