Study the Effective Factors on Aqueous Two-Phase Systems Containing PEG4000 and Phosphate Salt in Extraction of Malic Acid

Novel separation procedures that are essential for the purification of biomolecules in biotechnology industries, are developing. Hence, these methods should be rapid, selective, and easy to scale up. The aqueous two-phase system is an efficient method for the purification of materials. The purpose of the present work was to investigate the various parameters such as temperature, pH, salt concentration, and salt type in the polymer/salt system. In this research, purification of malic acid in the aqueous two-phase system which is containing polyethylene glycol 4000 g/mol and three variant salt (dipotassium hydrogen phosphate, diammonium hydrogen phosphate, and disodium hydrogen phosphate) were studied. According to the results, the optimum D and Y%  for extraction of malic acid was identified 0.85 and 80%, under these conditions, including 25% (w/w) disodium hydrogen phosphate at T=25°C and pH=5, respectively.