Preparation of a diabetic model of zebrafish (Danio rerio) by direct addition of glucose monohydrate in water

The aim of this study was to investigate the possibility of hyperglycemia in Zebrafish by adding glucose monohydrate directly to the water of its habitat. A group of 60 Zebrafish were collected from stock tanks of Zebrafish lab core facility in Endocrinology and Metabolism Research Institute (EMRI), and randomly divided into 6 tanks in two treatments with glucose and without glucose (control) in three replicates for each treatment. In the treatment containing glucose, the concentration of glucose monohydrate in the water of incubators was increased from zero to 72 g in two liters (36 g / l) of tank water for 9 days. Then the fish were kept and fed for three weeks in the mentioned conditions and at the end of the period, the blood sugar level was measured by cutting off the caudal peduncle. The results showed that the treated fish containing glucose monohydrate had 22.4% higher blood glucose than the treatment without glucose at the beginning of the experiment. At the end of the experiment, after 21 days of exposure to glucose monohydrate, the blood sugar level of fish affected by glucose monohydrate was measured 24.3% higher than the control treatment. Blood glucose levels in both treatments at the beginning of the experiment compared to the end of the experiment (after a period of 21 days) showed a statistically significant difference (p <0.05). According to the results of this study, the method of directly adding glucose monohydrate to make a model zebrafish for diabetes research can be achived.