Effect of combination of 2, 4-dinitrophenol as a targeted antioxidant and luteolin as a non-targeted antioxidant on functional parameter of rooster sperm during chilling storage at 4°C

In the process of sperm storage, production of reactive oxygen species (ROS) and reduction of antioxidant activity cause sperm damage. The most damage related to high ROS production and inadequate antioxidant penetration into mitochondria attacks this organelle. Purpose Combination of antioxidants was used to reduce oxidative damage. This study investigated the effect of different levels of combination of targeted antioxidant 2, 4-dinitrophenol and non-targeting luteolin on semen dilution on sperm parameters under in vitro storage at 4°C for 48 h. Semen samples were collected from 15 rooster at 28 weeks of age. After initial evaluations, semen samples were mixed with each other. After diluting the samples and adding different levels of antioxidants, treatment 1 (0.5 nM 4,2-di-nitrophenol + 1 μM luteolin), treatment 2 (0.5 nM 4,2-di-nitrophenol +3 μM luteolin), treatment 3 (0.75 nM) 4,2-Ditrophenol + 1 μM luteolin), 4 (0.75 nM 4,2-Dithinrophenol +3 μM luteolin) from combined antioxidant and storage of samples at 4°C for 48 h in refrigerator quantitative parameters were evaluated at 1, 24, 48 h after storage at 4°C. The parameters evaluated had a significant decrease over time. But the samples treated with the combination antioxidant significantly increased (P <0.05) in the measured parameters independent of the time and the interaction effect of the treatment on time. Also, addition of 3, 4, 5 and 6 treatments significantly decreased malondialdehyde concentration and treatment's 6 significantly decreased unhealthy spermatozoa (P <0.05).