Effect of irrigation regime on cotton golestan genotype (Gossypium hirsutum) yield, fiber quality and insilico analysis of CesA and XET1 genes involved in fiber quality

Study of water stress effect in traits of crops is related to stress tolerance, increasing their growth and yield in stress situation.This research was performed to evaluate the impact of different irrigation regimes on cotton yield, fiber quality were conducted using a split-plot factorial design with four irrigation levels (rainfed, 33%, 66% and 100%) as the main factor. After physiological maturation, fiber performance and quality traits were evaluated.protein and phylogenetic properties have been evaluated by bioinformatics tools. The results indicated that the highest yield with 1.2 kg was observed in 66% irrigation treatment. The highest fiber weight was observed in 66% treatment and the lowest in rainfed conditions. Seeds grown under 66% irrigation saved more water and produced potential seeds with high quality fibers. Bioinformatics analysis also revealed that the intracellular locations of CesA and XET1 enzymes are plasma membrane and cell wall, respectively. The enzymes CesA and XET1 belong to the protein family of transferases and hydrolase, respectively. Phylogenetic analysis revealed that the sequences of both cotton enzymes with co-family genera were separated in the Malvaceae . It appears that in order to grow cotton seeds with desirable fibers in low water conditions and in dry areas, it is better to use seeds that are irrigated under optimal irrigation conditions (66%). by cultivating cotton seeds with about one third of the water requirement in three years, in addition to reducing the amount of water requirement and irrigation water consumption, better yields can be achieved.