Comparison of the use of lactoferrin in combination with feed and sprays in physiological responses of yellowfin seabream (Acanthopagrus latus)

The aim of this study was to compare the effects of dietary lactoferrin (LF) in combination with feed and sprays on hematological factors and non-specific immune response of yellowfin seabream (Acanthopagrus latus) with an average weight of 10±0.3 g. This study was carried out in a completely randomized design with three treatments and replications in fiberglass tanks with 300 liters volume. Fish were fed with feed containing 0 and 800 mg lactoferrin spray and mix per kg feed for a period of 35 days. At the end of the experiment, blood and plasma samples were collected. The obtained results indicated that dietary LF did not change yellowfin seabream non-specific immune response except for lysozyme activity (P>0.05). TIBC and iron plasma significantly vary in fish fed on 800 mg spray and mixed lactoferrin respectively (P<0.05). Hemoglobin value and red and white blood cells count showed significant differences between the control groups with 800 mg mixed lactoferrin (P<0.05). Although hematocrit value was influenced by spray lactoferrin (P<0.05). Overall, this study showed that hematological and biochemical factors were affected by dietary lactoferrin. Nonetheless, it can be concluded that feeding of yellowfin seabream on the diet supplemented with 800 mg spray lactoferrin for a period of 5 weeks enhance the non-specific immune response.