Association between miR146a gene rs2910164 polymorphism with breast cancer in Irainian female by Tetra-Arms PCR

Gene expression is regulated with miRNAs, that are non-coding small RNA molecules and genetic variations such as miRNA genes SNPs, which are molecular biomarkers in the prediction of cancers risk. One of miRNAs that is associated with BRCA1 / BRCA2 genes, is miR146a and related with many cancers, including breast cancer. This research aim was association study between prevalence of rs2910164 SNP in miR146a gene with breast cancer in Iranian female population.

This study was performed on 50 patient and 50 control blood samples. DNA was extracted of samples. Then for genotyping was used Tetra Aarms PCR and results was statistically analyzed.Results- Frequency of genotypes for GG, GC and CC in control groups were 30%, 66% and 4%, and for patient groups was 46% , 52% and 2%, respectively. No significant difference was observed between frequency of dominant and recessive allels in control and patients gorups (P > 0.05).

In this study results, there was not significant difference between G and C alleles in the control and case groups. Therefore, could be indicated that there was not association between of miR146a gene rs2910164 polymorphism and breast cancer in the studied Iranian females population. So, further studies with larger sample size are required to support finding of this research.