Bioinformatics analysis of CBL gene family members in Sesamum indicum under drought stress

Calcineurin B-like proteins (CBLs) are a subfamily of calcium sensors that play a role in various plant cell processes and molecular functions. In sesame (Sesamum indicum), in silico analysis of the CBL gene family was performed to identify CBL proteins involved in calcium signaling. Using their orthologic relationships with Arabidopsis homolog genes, the nine SiCBL genes were identified and subdivided into six groups: SiCBL1, SiCBL2, SiCBL3, SiCBL4, SiCBL8, SiCBL10. The molecular weight of SiCBL proteins ranged from 24.4 to 37.9 kDa, the Isoelectric acid pH range, the instability index ranged from 33.99 to 47.46 percent, the aliphatic index ranged from 80.29 to 10.89, and the GRAVY ranged from -0.420 to 0.061. Prediction of post-translational modifications revealed that palmitoylation motif was observed in all siCBL, however majority of them did not have myristoylaton motif. In term of gene structure, 11% of SiCBL genes had nine exons, 11% had eight exons and 77% had seven exons. The RNA-seq pattern of the SiCBL subfamily under PEG treatment revealed that, whereas members of this gene family had generally similar expression patterns in both susceptible and tolerant cultivars, due to functional Convergence, each member of this gene family had a distinct expression pattern. Future research on the expression of SiCBL and SiCIPK gene family genes under various abiotic conditions could aid in understanding the mechanism of expression control of SOS-related genes.