Synthesis of Nanoscale Xerogel/MTX and Study Its Effects on the Liver and Kidney Tissue and Level of IgG in Rats with Rheumatoid Arthritis

The current study aims to reveal the therapeutic effect of Xerogel nanocomposite prepared and diagnosed on liver and kidney tissues and the level of rheumatoid factor(RF) concentration in rats induced by Complete Freund Adjuvant(CFA)  with rheumatoid arthritis(RA). Twenty male albino rats were randomly divided into four groups, each group containing 5 rats treated for 40 days: group 1 the negative control group, group 2 in which rheumatoid arthritis(RA) was induced by( CAF) by injecting 0.5 ml of the substance into the sole of the foot. Right was a positive control group, group 3: rats dosed with methotrexate (MTX) 125 mg/250g, group 4: rats dosed Xerogle/MTX 0.125mg/250 mg, liver and kidney parts were stained with hematoxylin-eosin and the immune cytokines of rheumatoid factor(RF) immunoglobulin protein IgG were measured. The results of the current study showed that the induction of rheumatoid arthritis(RA) by CAF led to changes in the tissues of the liver and kidneys through necrosis and hemorrhage in their tissues, as well as an increase in the level of rheumatoid factor (G2) concentration, and that treatment with (MTX) 125 mg / 250 g (G3) led to restoration The damages that occurred after the induction of rheumatoid arthritis and reduced this rise significantly. Also, treatment with the nanocomposite Xerogel/ MTX 0.125 mg / 250 g (G4) also led to a significant reduction in the level of IgG concentration in albino rats by reducing damage and inflammation in liver and kidney cells. The study indicated that treatment with Xerogel nanocomposite loaded with MTX increases the protective effect against the harmful effect of CFA-induced rheumatoid arthritis(RA) in liver and kidney tissue and lowers the immune parameters represented by rheumatoid factor(RF) IgG.