Pathomorphological Changes of Macrophage and Adipose Tissue Change and Negative Energy Balance Methods in Unsaturated-High-Fat-Fed Male Obese Rats of Mesenteric and Retroperitoneal Areas to Food Content

The aim of present study was to investigate the effect of 10-weeks of 4 energy restriction methods on histologic changes of adipose tissue and macrophage infiltration as well as glycemic indices in unsaturated-high-fat-fed obese male rats.

 Blood and tissue samples were collected from 72 wistar male rats (weight 195±5g, age 8 weeks) at 1st, 18th and 28th weeks after overnight fasting. After first sampling, remaining 64 rats were randomly divided into two groups of control (n=16) and high-fat-diet (UHFD)(n=48). UHFD group was randomly divided into food content change (SD), exercise training (EX), combination (CR+EX) and unsaturated-high-fat-diet (UHFD) subgroups after 18 weeks. Ex group ran for 10 weeks (5d/wks.) with intensity equivalent to 70-75% of Vo2max. CR was implemented reducing 25% of energy intake and SD group used a change from UHFD to a standard diet. Histologic changes of retroperitoneal and mesenteric regions as well as macrophage infiltration were assessed after tissue passage, H&E staining and high-resolution microscopic picturing.

The results of this study indicated that in comparison to UHFD, all experimental groups showed a significant difference in all glycemic indices and lipid profile variables (but HDL) as well as adipocyte size of studied adipocyte tissues (P > 0/05).

Therefore, it seems that regardless of the intervention type, energy intake manipulation methods possibly due to the reduction of adipocyte size had not change in macrophage infiltration to cause improvement in metabolic profiles.