Qualitative and Guantitative Changes of Organic Acids in Processed Olives by HPLIC

Four cultivars of olive, Kalamata, Marri, Zard and Fishmi were obtained from Roodbar town and treated with two methods, natural and controlled fermentation with Lactobacillus plantarum</em> as a starter culture at 25°C for 100 days. HPLC analysis experiments were conducted during the whole study period. A higher level (1.08% w/w) of total acidity was produced in Fishmi cultivar especially in the natural fermentation (0.8-1.1 g lactic acid/ml) than in other cultivars. Individual organic acids were determined by HPLC. Quantitative changes of organic acids in olives showed (P<0.0l) an increasing pattern of lactic and acetic acids during fermentation, but decreasing levels of malic and citric acids were revealed. The highest level of lactic acid (1.1% w/v) was observed in Zard cultivar and the type of fermentation had no effect on acid production. Malic and citric acids gradually disappeared during 50 days of fermentation period and this change was faster for citric acid during controlled fermentation. Malic acid did not follow this pattern.