investigate the effect of aerobic exercise with and without BFR on mitochondrial dynamic (MFN2 &DRP1) and mitophagy proteins (PARKIN &BCL2) of human skeletal muscle

The purpose of this study was to investigate the effect of aerobic exercise with and without BFR on mitochondrial dynamic and mitophagy proteins of human skeletal muscle. In a cross-sectional study, five young men (age: 33.4 ± 2.30 year, weight: 79.64 ± 10.49 kg, BMI: 26.24 ± 2.27kg/cm2) performed two aerobic exercise intervention with BFR (EX+BFR) and without BFR (EX) in two separate days. The aerobic exercise included 5 bouts of 2-min activities, interspersed by 1 min active rest which performed with and without BFR. Western Blot method was used to measure the protein levels of MFN2, DRP1, BCL2 and Parkin of skeletal muscle. Data analysis was performed using T test at a significant level of 0.05. The results of the data analyze showed that both EX+BF and EX significantly increased MFN2 and Parkin and also significantly decreased DRP1 compared to the pre-test (p < 0.05). However, EX+BF resulted in a significant increase protein levels of BCL2 compared to the pre-test (p < 0.05). Also, EX+BF has a significant effect on MFN2, BCL2 and Parkin elevation and DRP1 decrease in compared to EX (p < 0.05). Based on the findings of this study, it seems that aerobic exercise with and without BFR is a strong stimulant for the improvement of mitochondrial dynamics and mitophagy of skeletal muscle.