Protective effect of Iranian native probiotic mixtures (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus) on induced lesions of lead acetate in lung tissue of male rats

Probiotics are living microorganisms that, when consumed in sufficient quantities, have beneficial effects on the host, and their excellent characteristic is that they are safe, secure, and have antioxidant activity for the host. The aim of this study was to investigate the effect of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus helveticus, and Lactobacillus casei) on lung tissue changes in male rats infected with lead acetate. In this experimental study, 21 male Wistar rats were divided into 3 groups of 7: control, lead acetate, lead acetate + mixture of native Iranian probiotics. A single dose of lead acetate injection (10 mg/kg) was done intraperitoneally and probiotics (109 CFU/ml) were gavage for 35 days. After the end of the experiment, the rats were anesthetized, then tissue sections were prepared from the lungs, the samples were stained with hematoxylin-eosin and photographed and studied by a light microscope. The study of morphological changes in lung tissue in the group treated with probiotics showed a reduction in tissue damage compared to the lead acetate group. According to the findings of this study, the use of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus helveticus, and Lactobacillus casei) can improve the induced damage of lead acetate in lung tissue.