Histopathological evaluation of topical autologous platelet-rich fibrin in femoral head and neck ostectomy in rabbits

This study was to evaluate the histopathological changes following topical administration of PRF in femoral head and neck ostectomy (FHO) in rabbits. 20 New Zealand white rabbits underwent FHO. In the treated group, surgical site filled with PRF. After two and four weeks, all animals were euthanised and femur was cut approximately 2 cm far from the head of femur. For microscopic study, some slides were prepared from samples through serial sectioning and were stained with H&E and Mason Trichrome. Qualitative changes such as granulation tissues, cartilage and bone formation and their timeliness, thickness of collagen fibers and cellular changes and differences were observed and noted. Average occupancy level of each tissue and their ratio to whole surface of sample and to each other in two groups were calculated and compared. The results of this study shows that in PRF treated group, ratio of cartilage tissue formation to granulation tissue as well as formation of bone to granulation tissue is significantly more than these parameters in control group. This result indicates to faster healing on the damaged area in treated group. Also in this Group cartilage and bone tissues formation in the healing process was more orderly. The results indicate that the use of PRF has been able to accelerate healing of bone tissue that is cut at the head of the rabbit’s femur. So it seems that the use of PRF as a treatment protocol to accelerate the healing period of bone tissue in these cases could be suggested.