Interspecific gynogenesis induction in Siberian sturgeon (Acipenser baerii ) using heterologous sperm
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Siberian sturgeon (Acipenser baerii) grows rapidly in breeding ponds. Gynogenesis is a suitable technique for producing sturgeon female sex. The aim of this study was to establish female sex in Siberian sturgeon using induction of gynogenesis. The sperm of Persian sturgeon (Acipenser persicus) was genetically inactivated by UV radiation at a dose of 8514 J/m2. In this case, sperm with 40% motility were combined with Siberian sturgeon oocytes and in 2°C cold shock for 30 minutes, 10 minutes after activity, gynogenic progeny were created. Fertilization rate was 44.5% (control hybrid 62%) and hatching rate was 19.8% (control hybrid 40.5%). Purely maternal heritability in gynogeneic progeny was confirmed using microsatellite markers. By achieving the biotechnique of sperm production with inactivated DNA of Persian sturgeon, it is possible to prevent the risk of extinction and protect other sturgeon by inducing gynogenesis.
Keywords:
Language:
Persian
Published:
Physiology and Aquatic Biotechnology, Volume:10 Issue: 3, 2023
Pages:
23 to 48
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