Grouping of some soybean genotypes in various salt stress treatment in Germination Stages and Study of Genetic Diversity using Molecular Markers

In the present study, 11 ISSR, 2 Retrotransposon and 15 Retrotransposon + ISSR composed markers (REMAP) were evaluated among 27 genotypes of Soybean. 130 polymorphic bands were produced from 28 primers. The maximum percentage of polymorphism were 77% for UBC808, 54% for TOS-2 and 70% in UBC808+TOS-1 marker. The mount of Nei′s, Shanon and PIC index for UBC808, UBC811, UBC825, HB-12, TOS-2, UBC807+TOS1 and UBC834+TOS-1 indicated that theses markers could be used to assessment of genetic variation. The result of cluster analysis using complete linkage algorithms clustered 27 studied genotype in 4 distinct groups with 4, 9, 9 and 5 genotype in respected group. Discriminant function analysis using the Fisher′s linear method showed that the complete linkage method separated the genotypes with 96/3 percent accuracy. Morphological characteristics of genotype were evaluated in a completely randomized design with factorial arrangements whit two factors, The first factor were salt stress treatments at 0 (check), 30, 60 and 90 mM NaCl and the second factors was genotypes. ANOVA showed significant differences among varieties and level of Treatment for all measured morphological traits, and in interaction of cultivar×stress for all traits except Shoot fresh weight, shoot dry weight, root dry weight and shoot's weight difference between wet and dry. The cluster analysis based on within group′s method clustered genotype at different salt stress treatment in 4 clusters, and the accuracy of cluster analysis revealed by discriminant analysis was 92/6%. The correlation between the two similarity matrices was not significant by Mantel test. Analysis using marker-assisted selection of bands that enhance salt tolerance traits mean they can be used to identify resistant soybean genotypes..