The Effects of Aerobic Exercise and Ginger Extract Loaded into Chitosan Nanoparticleson Atrial Natriuretic Peptide (ANP) Gene and Cardiac Histopathology in Rat Models of Myocardial Infarction

In this study, the effects of aerobic exercises and encapsulated ginger extract (GE) into chitosan nanoparticles (CNPs) were investigated on atrial natriuretic peptide (ANP) gene expression and cardiac histopathology in rat models of myocardial infarction.

The 25 male rats were divided into five groups: Isop (85 mg/kg), Isop + CNPs, Isop + GE-CNPs, Isop + AE, and Isop + GE-CNPs + AE. The rats performed AE on a rodent treadmill five days per week for six weeks. GE-CNPs were gavaged to rats at a dose of 500 mg/kg for six weeks. After the end of the treatment period, the expression of ANP genes was carried outby Real-Time PCR.

Based on our findings, the highest amount of collagen deposition was in the heart tissue of patient groups and the patient group treated with chitosan nanoparticles (P<0.05). Besides, the results of real-time PCR showed that the changes in ANP gene expression were significant only in the groups treated with aerobic exercise and ginger extract (P<0.05).

Regular aerobic exercises with nano-drug supplements such as ginger extract encapsulated into CNPs can reduce cardiac fibrosis and regulate ANP gene expression.