Angiotensinogen Gene M235T and T174M Polymorphisms in Diabetic Nephropathy in a Bangladeshi Population

Marker gene polymorphisms linked with the renin-angiotensin-aldosterone system (RAAS) have been broadly studied in diabetic nephropathy (DN) patients considering that RAAS is a potential drug target to slow down kidney disease progression.

The aim of the present study was to determine the link between M235T and T174M variants of angiotensinogen (AGT) gene and DN.

A total of 93 patients with DN, mean age of 56 ± 8 years, systolic blood pressure (SBP) of 141 ± 14, and diastolic blood pressure (DBP) of 84 ± 7 mm Hg (mean ± SD) were investigated, among whom 59 patients had a family history of type 2 diabetes mellitus. A total of 96 healthy subjects served as the control group with no family history of diabetic nephropathy (FHDN) and type 2 diabetes mellitus, a mean age of 47 ± 10 years, SBP of 126 ± 11, and DBP of 76 ± 6 mm Hg. PCR–restriction fragment length polymorphism was employed for genotyping M235T and T174M molecular variants.

Genotype frequencies of the variants M235T (χ2 = 2.038, P = 0.361) and T174M (χ2 = 2.952, P = 0.229) did not show any statistically significant association with type 2 diabetic nephropathy (T2DN) compared to the control. Based on FHDN and family history of diabetes mellitus (FHDM), the frequency of genotypes of M235T marker (P = 0.360) in FHDN, and (P = 0.886) FHDM; T174M marker (P = 0.641) in FHDN, and (P = 0.425) FHDM also did not show any statistically significant association with T2DN compared to the controls.

M235T and T174M variants were not associated with DN in a Bangladeshi population.