Investigation on the Antibacterial ‎Features of Mucus in Boleophthalmus ‎Dussumieri Mudskeeper

Due to the difference in the quality and quantity of proteins and enzymes in the mucus of different types of fish, therefore they have different antibacterial activity. Boleophthalmus dussumieri fish is one of the mudskeeper’s species that has been reported from Musa Estuary according to previous studies. Therefore, the aim of this research is to investigate the antibacterial effect of B. dussumieri fish mucus against 3 standard bacterial strains (Escherichia coli (ATCC 25922), Acinetobacter baumannii (ATCC BAA-747), Bacillus subtillis (ATTC 6633)) and 3 clinical bacterial strains (Escherichia coli, Acinetobacter baumannii, Bacillus anthracis). After preparing the aqueous extract of mucus, its antibacterial effect was investigated in four concentrations of 25, 50, 100 and 200 mg/ml by disc diffusion method. Data were analyzed using SPSS 21 software and one-way ANOVA. The results showed that all the bacteria were sensitive to the mucus extract and there was a significant difference (P<0.05) in the growth inhibition zone between different bacteria. It was also found that Gram-positive bacteria with an average non-growth zone of 12 mm showed more sensitivity to B. dussumieri fish skin mucus extract than Gram-negative bacteria with an average non-growth zone of 9 mm. The results of this research showed that the skin mucus of Boleophthalmus dussumieri fish has antimicrobial effect and has the ability to remove pathogenic agents in laboratory conditions.