Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin
Author(s):
Yan Jiang , Linqiao Li , Qilu Pan , Xiaojing Du , Qian Han , Feixiang Ling , Rou Li , Lin Mai , Jianwei Huang , Shuyuan Chu , Libing Ma *
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background
Little is known about MBD2’s epigenetic regulation in the immune pathogenesis of CD4+T cell differentiation.Objective
This study attempted to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen ovalbumin (OVA).Methods
Mononuclear cells were separated from the spleen tissues of male C57BL/6 mice. The OVA interfered with the differentiation of splenic mononuclear cells and CD4+T cells. The CD4+T cells were obtained by magnetic beads and identified by CD4 labeled antibody. CD4+T cells were transfected with lentivirus to silence MBD2 gene. A methylation quantification kit was used to detect 5-mC levels.Results
The purity of CD4+T cells reached 95.99% after magnetic beads sorting. Treatment with 200 μg/mL OVA stimulated the CD4+T cells differentiation to Th17 cells and promoted the secretion of IL-17. After being induced, the Th17 cell ratio increased. 5-Aza inhibited the Th17 cell differentiation and the IL-17 level in a dose-dependent manner. Under the intervention of the Th17 induction and 5-Aza, MBD2 silencing inhibited the differentiation of Th17 cell, and decreased the IL-17 and 5-mC levels in the cell supernatants. MBD2 silencing reduced the scale of the Th17 cell and IL-17 levels in the OVA-treated CD4+T cells.Conclusion
MBD2 affected IL-17 and 5-mC levels by mediating the Th17 cell differentiation in splenic CD4+T cells that were interfered with 5-Aza. OVA induced Th17 differentiation and increased IL-17 levels, inhibited by MBD2 silencing.Keywords:
CD4+T Cell , IL-17 , MBD2 , Ovalbumin , Th17 Cell
Language:
English
Published:
Iranian journal of immunology, Volume:20 Issue: 1, Winter 2023
Pages:
45 to 56
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