Antimicrobial effect of silver particles synthesized from hydro alcoholic extract of Urtica dioica on Staphylococcus aureus and Pseudomonas aeruginosa

Nano-scale biological compounds that have unique physical and chemical properties have become effective in recent years. Today, due to the cost of production and compatibility with the environment, the use of extract in the synthesis of nanoparticles has received attention. The present study was conducted with the aim of investigating the antimicrobial properties of silver nanoparticles synthesized from the hydroalcoholic extract of Urtica dioica on Staphylococcus aureus and Pseudomonas aeruginosa.In this experimental study. First, the hydroalcoholic extract of Urtica dioica nettle extract was prepared, then, by adding silver nitrate salt with a concentration of mM to the extract, the reaction was carried out at room temperature and after the color change of the reaction solution containing nanoparticles, the physical and chemical properties of silver nanoparticles synthesized using From SEM, XRD and UV-vis was studied. Antimicrobial effect of silver nanoparticles with different concentrations was evaluated by MIC method.The maximum absorption of silver nanoparticles was recorded at a wavelength of nm. The average diameter of the synthesized nanoparticles was the results of the antimicrobial test showed that the synthesized silver nanoparticles have a positive effect on both bacteria. The MIC concentration of nanoparticles for Staphylococcus aureus was μg/ml and for Pseudomonas aeruginosa μg/ml.It seems that silver nanoparticles synthesized with the hydroalcoholic extract of Urtica dioica have antibacterial activity and can probably be used as an antimicrobial candidate.