Isolation and identification of microbiota of Culex quinquefasciatus for their application as paratransgenic tools in vector control

Although the study on the bacteria residing in the mid-gut, salivary gland, and reproductive organs of insect vectors have drawn appeal to the host-pathogen interactions, we know comparatively less about microbiota that naturally exist in different mosquito organs within Iran.

In the current investigation, PCR assay by using 16S rRNA gene amplification and DNA sequenc- ing, in addition to the traditional culture-based approach utilized for the detection of cultivable bacterial assemblages in mid-gut and reproductive tracts of Culex quinquefasciatus.

The identified bacteria isolated from different tissues of 45 individuals were consisted of Achromobacter, Aero- monas, Arthrobacter, Asaia, Enterobacter, Gluconobacter, Klebsiella, Lysinibacillus, Micrococcus, Psuedomonas and Serra- tia. The results showed that Proteobacteria was the most prevalent phylum in both genders' mid-gut and reproductive tracts, and Asaia was the most common bacteria that originated in adult females and males’ tissues.

These outcomes recommend that the discovered microbiome may span through Cx. quinquefasciatus popula- tions. This data can be utilized to interfere with the transmission of pathogens and design new strategies for the control of mosquito-borne diseases.