Synthesis of Silver Oxide Nanoparticles using Different Precursor and Study Cytotoxicity Against MCF-7 Breast Cancer Cell line

In this work, the eco-friendly synthesis of silveroxidenanoparticles (Ag2O-NPs) was successfully performed using orange leaf extract with silver nitrate and silver sulfate as precursors. Several techniques were used to characterize the surfaces of the silver oxide nanoparticles (Ag2O-NPs), including FT-IR spectra, which showed the functional groups present in the synthesis of silver oxide nanoparticles, FE-SEM images showed that the green synthesis of Ag2O-NO3-NPs and Ag2O-SO4-NPs has a spherical shape. EDX analysis spectrum confirmed the presence of elemental silver signals in the Ag2O-NPs, proving that the Ag2O-NPs were prepared within the nanoscale range and that there is a clear and tangible effect of negative ions bonded (NO3- and SO4-2) with Ag metal on the size, shape, and distribution of Ag2O-NPs. Also, the cytoxicity of the established Ag NPs at 0-200 µg/ml was assessed in breast cancer MCF-7 cell lines in vitro using MTT assay, and verified these strong cytotoxicity. As a result, the use of orange leaf extract is considered an eco-friendly and cost-effective method.