Producing Embryoid Bodies from Embryonal Stem Cells Using Siliconized Petri Dishes

Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques have been used to obtain EBs, the improvement of the technique of EBs formation can help in achieving better results in ESCs differentiation into neurons, myocardiocytes, hemopoietic cells and others. In this investigation Sigmacote was used as a hydrophobic substrate to improve EBs formation. Petri dishes` floor was been siliconized by a three-step procedure and with sigmacote. Then CCE and p19 cell line were used to obtain EBs in these siliconized petri dishes. These cells were transmitted to petri dishes, the concentration of them was 1105 cells/ml, after 48 hours embryoid bodies were counted. Control group was bacteriologic petri dishes with same conditions. The results of EBs calculation showed that siliconized petri dishes in comparison with bacteriological petri dishes can be produced EBs with high efficiency and statistically it was significant (p<0.05). Results of this research indicated that sigmacote as a hydrophobic substrate can improve EBs formation by an easy and reproducible method. Therefore it is suggested to increase EBs formation and differentiation of ESCs.