The Effect of 4 Weeks Aerobic Exercise Training with Detraining Courses in Various Prevention Phases on BCl‑2 and BAX Genes Expression and Proteins

The aim of this study was to investigate the effect of aerobic exercise with detraining in different phases of prevention on BCL2 Associated X (BAX) and B-cell lymphoma 2 (BCl‑2) gene expression and proteins.

For this purpose, 32 female Balb‑c mice (18–20 g) were purchased and randomly assigned to primordial prevention (A), primary prevention (B), secondary prevention (C), and control (D). A group performed aerobic exercise for 4 weeks, after 4T1 cells injection detrained for 8 weeks. Group B performed aerobic exercise for 4 weeks immediately after injecting 4T1 cells and then detrained for 4 weeks. In C group, the 4T1 cells were first injected and did not perform any activity for 4 weeks, followed by 4 weeks of aerobic exercise. Forty‑eight hours after the last training session and detraining courses, after anesthesia, sacrificing, and tissue removal, were performed. Reverse transcription polymerase chain reaction (RT PCR) was used to measure gene expression and Western blot (WB) was used to measure protein content. One‑way Analysis of variance (ANOVA) test was used to analyze data.

The results showed that aerobic exercise in A, B, and C groups compared to D group reduced BCl‑2 gene expression and protein and increased BAX gene expression and protein.

Therefore, exercise can cause apoptosis in tumor cells by increasing pre‑apoptotic factors and decreasing antiapoptotic factors in tumor cells, and consequently improving the disease status.