Optimizing Parameters for Bio-Hydrogen Production from Mixed Culture and Food Wastewater

In this research, Rice Boiling Wastewater (RBW) has been used as inexpensive food wastewater for bio-hydrogen production by dark fermentation. The effective parameters such as temperature, pH, time reaction, and substrate concentration have been optimized.  The 2k-1 factorial design method and response surface methodology were used to optimize the best reaction conditions. According to the results, under optimal conditions pH (6.240), temperature (35.090°C), sugar concentration (5.400 g/l), and fermentation time (36 h), the highest hydrogen production in the proposed theoretical model was obtained equal to 3.30 mol H2/mol glucose. The proposed theoretical model demonstrated a good agreement with the obtained experimental data.