Synthesis of thiolated nanochitosan as a carrier of biperidine using two cross linkers Tripolyphosphate (TPP) and Genipine and evaluation of their release and toxicity

Modern science in drug delivery systems has long been paid to effectively design drug delivery systems, to reduce side effects, increasing bioavailability, targeted drug delivery, and passing through blood-brain barriers. Nanoparticles are very important as carriers because they  carry different types of drugs to different parts of the body at the right time.

In the present study, thiolated nano chitosan loaded with Biperiden was synthesized by ion gel method using two types of crosslinkers (tripolyphosphate and genipin) and their efficiency was investigated.

FTIR analysis confirmed the successful synthesis of various stages of thiolated nano chitosan loaded with Biperiden by both crosslinkers. SEM images showed that as-synthesized nano-carriers have a nanorod structure and nanocarriers crosslinked by genipin had a more regular structure with a size of about 150 nm than nanocarrier crosslinked by TPP with a size of approximately 200 nm. In vitro drug release and cytotoxicity studies showed nano-carriers crosslinked by genipin have had a higher release and less cytotoxicity than nano-carriers crosslinked by TPP.

Considering the lower toxicity and delayed release of nanoparticles synthesized with genipine than nanocarriers synthesized with TPP, the use of this nanocarrier increases the bioavailability of the drug and can be used as a suitable drug delivery system.