Evaluation of antibiotic resistance and frequency of sea gene in Staphylococcus aureus isolated from clinical sources in Karaj

Infections caused by Staphylococcus aureus are on the rise due to the spread of resistant strains, an increase in immunocompromised patients, and the overuse of medical equipment. The bacterium is also an opportunistic pathogen that provides conditions for invading the host by producing and secreting various toxins, including various enterotoxins.The aim of this study was to evaluate the antibiotic resistance and frequency of sea gene in Staphylococcus aureus isolated from clinical sources in Karaj.
 

This descriptive-observational study was performed on 100 Staphylococcus aureus isolates collected from clinical sources in Karaj. Collected samples were transferred to the laboratory under appropriate conditions by culture, microscopic and biochemical methods and then the susceptibility of isolated bacteria to various antibiotics was measured by disk diffusion agar method according to CLSI (2019) instructions and finally to test production ability. Enterotoxin A, the presence of sea gene was assessed by PCR

Based on the results of biochemical tests, 100 isolates of Staphylococcus aureus were identified. According to the antibiogram test, these bacteria had the highest resistance to penicillin with 92% and ceftazidime with 82% and also 100% of the isolates were sensitive to vancomycin. The results of PCR reaction also showed that out of 100 isolates, 79% contained sea genes.

Considering the importance and role of Staphylococcus aureus in the pathogenesis and development of nosocomial infections, drug resistance and the presence and expression of enterotoxin genes in clinical specimens of this bacterium should be considered in disease control management.