Preparation of Polyclonal Antiserum for potato X virus

Recently, as a result of the development of agriculture, and the expansion of the international export of seeds and seedlings, pathogenic viruses that infect potatoes have spread to other regions, and changes in viruses under the influence of various climatic factors have led to the emergence of strong pathogenic isolates. The preservation of potato viruses in potato nodules and other plant organs creates several difficulties in their diagnosis. For this, it is advisable to use highly sensitive diagnostic methods. In this work, the results of the research on the preparation of polyclonal serum, which is the basis for obtaining antibodies necessary for the immunodiagnosis of potato X virus (PXV), are given. In this case, an isotonic solution of NaCl or cycloferon together with a purified virus antigen was injected between the muscles of the biceps and hind legs of rabbits. The delivered antigen dose was increased by the same amount for both rabbits. Before reimmunization, the titer of sera from the rabbit injected with cyclotron was 1:64, the titer from the rabbit injected with an isotonic solution of sodium chloride was 1:32, and after reimmunization, the titer of the serum isolated from the first rabbit decreased to 1:32, and from the rabbit injected with NaCl the resulting serum was found to be elevated to a titer of 1:256. The prepared serum was used in the diagnosis of PXV and its amount in plant tissue was determined.