Study of genotype, cold pre-treatment, low-dosage Gamma irradiation and 2,4-D concentration effects on wheat anther culture response

In this study, effects of genotype, cold pre-treatment, low dosage Gamma irridiation and 2, 4-D concentration on response of three wheat genotypes (Atrak, F3 2005 and F3 2104) to anther culture were investigated. Seeds of donor genotypes were grown under field condition in early spring. Anthers from donor plants were collected and plated on modified CHB medium containing 2,4-D (2 and 4 mgl-1), 0.5 mgl-1 Kinetin and gl-1 Sucrose. Number of calli formed in 100 anthers and number of plantlet produced from 100 calli were counted. Results indicated that genotypes, cold pre-treatments and 2,4-D concentrations had significant effects on response of wheat genotypes to anther production, while F3 2104 the lowest. It would be concluded that androgenic traits are controlled by genotype and environmental factors. Furthermore this traits are controlled independently.