Saturating Microsatellite Linkage Map of Wheat in Fukuho-Komugi × Oligo-Culm Cross Population using AFLP Markers

Genetic maps with high genome coverage are becoming increasingly useful in both basic and applied genetic researches. In the last decades, the advent of DNA markers has brought about a magnificent revolution in the production of genetic map, especially in wheat. In the present study, AFLP markers were used to saturate linkage map of 107 doubled haploid individuals produced through Fukuho _Komugi × Oligo – Culm crosses received from Japan International Research Center of Agricultural Science (JIRCAS). The framework of genetic map was used as base map for next analysis. AFLP analysis was performed with MseI / PstI as digestive enzymes. The average percentage of polymorphism with AFLP markers was around 16.6%. Data analysis was performed by computer program known as Mapmaker / EXP, Ver. 3.3. In this program, the maximum distance criterion was 50 cM and the minimum LOD equated 3. The drawing of chromosome schema for the linkage groups was performed by Draw map, Ver 1.1. In this analysis, 115 AFLP markers were divided into 10 groups in addition, some of the markers remained unlinked. The supplementary data analysis along with specific SSR markers identified the chromosome loci of the markers. Ultimately, 71.1% of the markers were assigned to genome A, 16.5% to genome B and only 3% to genome D. The AFLP markers filled 11 gaps in 7 chromosomes (2A, 3A, 7A, 2B, 3B, 5B and 7B). The low coverage of genome D was due to the limited polymorphism and its conservation in different populations. Among the chromosomes, maximum number of markers (60) was assigned to the chromosome 7A. The distribution of the markers on this chromosome was not uniform. Such a distribution was related to the grouping AFLP markers within heterochromatin region, particularly around the centromere.