Plasma Creatinine, ATP and Glucose Concentrations, Energe Expenditure and Anaerobic Power Markers after two Consecutive RAST Tests Female College Students

The relationship between the tissue energy status, particularly ATP levels and power out put during muscular activity is well known. The same as ATP, plasma/urine creatinine concentration has also been considering as muscle mass status and renal function during exercise and at rest. The purpose of this study was to investigate plasma variables and energy expenditure in response to running based-anaerobic sprint test (RAST) in female college students. Twenty-six female physical education students (age 22/34±0/3 year; 160/2±0/84 cm, 55/65±1/6 Kg, 21/62±0/48 in BMI, and) volunteer to participate in the present study. Subjects were asked to perform two RAST tests which separated with 1 minute rest between. Blood sample were obtained from antecubital vein before and immediately after the end of second test. Plasma Creatinine and glucose were measured by enzymatic methods. ATP was extracted by alcoholic extraction method (96 ethanol/4 water vol), and measured by using a luciferine - luciferase ATP assay. A paired t-student was used for analysis, plasma creatinine showed a non-significant decrease. Glucose concentration was significantly (p<0/03) higher immediately after the tests. A reduction in plasma ATP levels was significant (P<0/007). In addition a significant decrease in Max power, average power and increased energy expenditure were observed. the results indicate that plasma ATP changes might be consider as a marker for energy status and RAST can be used a stimuli for metabolic responses such as similar test.