Effect of Acute Supplementation of 500 mg Vitamin C on Exercise-Induced Lipid Peroxidation and Inflammation

The aim of this study was to examine the effect of acute supplementation of 500 mg vitamin C on oxidative stress and inflammation after 30-min exercise at 75% Vo2max. Sixteen healthy male, unfamiliar with the exercise protocol, randomly received either antioxidant (AO) (500 mg vitamin C) or placebo (P) 2h before a 30-min treadmill run at 75% Vo2max. Plasma vitamin C concentration (VC) and Malondealdehyde (MDA) as oxidative stress marker using HPLC, Creatine Kinase (CK) as muscle damage marker using Autoanalzser, and Interleukin-6 (IL-6) as inflammation marker using ELISA, Were measured. ANOVA repeated measures with Bonferroni correction was used to compare results over the times and independent t-test was used to determine between groups differences. Vitamin C increased significantly 2h after supplementation, immediately and 2h after exercise in AO group (P<0/05).Serum CK, increased significantly after exercise in both groups (P<0/05) and decrease 24th after exercise only in OA group. Serum IL-6, increased significantly after exercise (P<0/05) and decrease 24th after exercise in both groups. MDA increased significantly after exercise in P group (P0/05).

Conclusion

The results indicate that 500 mg VC supplementation prevented muscle damage and exercise-induced oxidative stress but not inflammation in these subjects.