Effects of HMG & r-FSH on development of ovarian follicles of Balb/C mouse

Gonadotropins are routinely used in animals and humans to induce multiple ovulations and increase the number of available oocytes in some fertilization techniques such as IVF (In Vitro Fertilization). This study was performed to assess the effects of HMG & r-FSH on development of ovarian follicles of mouse Balb/c. In this experimental study 60 immature (21 days old) female Balb/c mice were allocated into 4 groups: 1- Control group, without injection, 2- Sham or exposed group, with normal saline injection, 3- HMG group received 7.5 IU/ml HMG, and 4- r-FSH group received 7.5 IU/ml r-FSH. After 48 hours, the animals were sacrificed and their ovaries removed for histological studies. The average follicles quantity, and also the size and weight of the ovaries were analyzed using Kolmogorov-Smirnov and uni factor variance analytic tests, respectively. Data showed that using r-FSH significantly (p<0.05) increased the size, but not the weight of ovaries and different types of follicles (p>0.05). Also using HMG significantly increased the weight of ovaries and number of primary antral follicles (p<0.05) but not the size of ovaries and primary, secondary and growing follicles (p>0.05). HMG & r-FSH did not have significant effects on primary development of ovarian follicles but HMG affect the late stage development of ovarian follicles.