The Frequency of Prevotella Species in Women with Bacterial Vaginosis and Detection of the Drug Resistance

Recently drug resistance to three species of Prevatella (P.bivia, P. melaninogemcus, P. disicns) is seen. The aim of this study was to evaluate the frequency of bacterial vaginosis and detection of drugs resistance of Prevotella species. This descriptive study performed from 2004 to 2005 at Fatemi Hospital of Hamadan, Iran, on 310 female patients with vaginal discharge. This study was approved by the local committee of medical ethics. Laboratory diagnosis of bacterial vaginosis was based on 4 criteria including study of the homogenesity of the discharges, performing of whiff test, search for clue cell in smear and determination of pH of the discharge. Antibiogram test was performed by method of Kirby-Bauer and 9 commonly used antibiotics were applied. The data collected in a questionnaire and analyzed, using spss software. Of 310 patients with discharge, 65.7% were diagnosed as bacterial vaginosis that 47.4% of them had clue cells, 66.2% were whiff test positive, pH of 87.1% of the discharge were more than 4.5 and 76.7% had non-homogenous discharge. Out of 310 samples, 60 (19.3%) Prevotella species isolated in which 80% was P.bivia, 11.6%P.disiens and 8.3% P.melaninogenicus.These isolates and 4 references strains were resistant to penicillin (100%) and to vancomycin, kanamycin (> 60%). Resistance to cefoxcetin, clindamycin, metronidazole and ciprofloxacin were less than 10%. The frequency of bacterial vaginosis in our patients was relatively high. The most frequent isolated strain was P.bivia and also the most effective antibiotics against Prevotella isolates were ciprofloxacin, cefoxcetin, clindamycin and metronidazole.