The Histological Study of the Effect of Hyaluronic Acid and Curcuma Longa-Ghee compound on the Gingival Healing following Gingivectomy in Dogs

Currently, hyaluronic acid is used to reduce the side effects of periodontal surgery including hemorrhage, inflammation and pain and accelerate the healing process of the wound. Oral or local application of Ghee can be relatively effective in enhancement of the wound healing process.The present study was performed with the aim of comparing the effects of hyaluronic acid with curcuma longa- ghee on the healing process of gingival tissue following ginivectomy.In a double blind controlled randomized experimental study, 5 male beagle dogs, 8 to months old (approximately 5 kg), with intact teeth and clinical signs of gingivitis with similar Gingival Index (GI) and no periodontitis, were used. In each Jaw, two regions were randomly selected as control and six regions as test groups. Following gingivectomy periodontal packs were used in the control groups and three materials including curcuma longa-ghee with two different ratios (material A and material B) and hyaluronic acid (material C) were used in the test groups Histological changes were evaluated and 7 days after gingivectomy in order to assess the healing process. Data were analyzed using Kruskal Wallis test.Significant statistical differences were found in all parameters for the three materials used in experimental groups compared with control groups. On the fourth day, the inflammatory parameters decreased and repairing parameters increased remarkably in the regions with materials A and B compared to those with material C, while the difference in the degree of epithelialization was statistically meaningful (p = 0.00). On the seventh day, the inflammatory parameters decreased and repairing parameters increased remarkably in the material B regions compared to the regions on which materials A and C were placed. According to the above results, curcuma longa-ghee is more effective than hyaluronic acid in repairing process and reduction of the inflammation. Therefore, it can be considered as a suitable substitute for accelerating of healing process after periodontal surgery.