In vitro plant regeneration from the callus of shoot tipsin cotton (Gossypium hirsutum L. cv. SVPR 2)

A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 glucose and 8 gl-1 agar. The highest rate of shoot proliferation was achieved at 12th week and 8.6 shoots were produced per callus clump. Among the different concentrations tested media fortified with MS salts, B5 vitamins, 2 isopentynyl adenine (2-iP) (1.5 mgl-1), gibberellic acid (GA3) (0.6 mgl-1), glutamine (25 mgl-1) and glucose (30 gl-1) showed best response for multiple shoot proliferation from the induced callus. All the in vitro regenerated shoots were rooted on the medium fortified with MS salts, B5 vitamins, 30 gl-1 sucrose, 8 gl-1 agar and 1.5 mgl-1 of indole butyric acid (IBA). The regenerated plantlets, with tertiary roots are considered as matured plantlets and they were hardened on paper pots containing sand, soil and vermiculite in 1:1:1 ratio. The hardened plants showed 85% survival rate and showed parental phenotypic characters.