lnvcstigationo f CalpastatinG eneP olymorphisma nd its Relationshipw ith Daity

Polyrnorphisrno f CAST scne was investigatedw ith 200 purebredG hezel sheepw hich were randomlyc ollectedfi 'ornG hczelb reedings tationo f MyandoabA. fter extractingo f genomicD NA, exon I o1'calpastatignc new ith 622 bp was amplifiedw ith specificp rimerc.T he SSCPt echnique wasu sedl br deleclingth e PCI{productsu singp olyacrilamidg el ands ilver stainingm ethod.I n this researchth reea llelesw ere observedh avingt he frequencieso f 0.62, 0.35 and 0.03,r espectively. Comparisono f allele frequenciesin dicatedt hat Ghezelp opulationw as not in Hardy-Weinberg equilibrinm.A verageh eterozygosityfo r Ghezels heepw as 37%. The result also indicatedt hat genotypch ada signilicante llecto n daily gainf rornb irth to six monthsw eight(P<0.05)T. herefore,the rcsultsc onlilmcd that polymorphisma t this genec an be a useful tool for selectionp rograms basedo nn arkcr-assistesde lection.