Culture and Expansion of Rat Mesenchymal Stem Cells Using the Serum Prepared from Rat s Peripheral Blood

The aim of this study is to evaluate the effects of prepared serum from rat peripheral blood on growth and viability of rat bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Bone marrow cells prepared from rat s tibia and femur central canal were cultured either in medium containing fetal bovin serum (FBS) or rat peripheral blood-derived serum (PBDS) for three successive passages during which viability growth and proliferation of the cells were evaluated by MTT [3- (4 5- dimethylthiazol-2-yl)-2 5- diphernyltetrazolium bromide] colony forming assay calculation of population doubling number and daily examination of the cell growth for drawing growth curve. Each experiment was performed ten times and the mean values were statistically compared. In this study third passaged cells were evaluated in terms of their bone and adipocyte differentiation potentials. Morphologically the cells cultured with PBDS appeared to be somewhat more spindle than of FBS cells. Comparatively these cells seemed to have clear cut border than of FBS-cultured cells. According to MTT assay the cells from PBDS group significantly showed more absorption value than of FBS group indicating that they would be more viable than FBS cells. Furthermore in this group more colon were formed and more population doubling number (about twice of FBS cells) occurred (p