Canary sex identification using genetic markers

In many bird species, the sex determination is relatively difficult in young birds and even adults. But the sex of birds and other animals were determined from the beginning of zygote formation. Nowadays, sex identification of animals in throughout of their lives is possible using molecular genetic techniques. In the present study, the sex identification of canaries by PCR methods base on CHD-Z and CHD-W genes were explained. Genomic DNA was extracted from feather bulb (old and young) of 29 canary. PCR reaction was performed by a set of novel primer. The results show that, two amplified fragments, about 345 and 306 bp for female, and only one 306 bp amplified PCR product for male canaries. This result was shown for each sample. This sex identification system presented for the first time, especially in canaries and all of the molecular results confirm that in morphological species of male and female birds. We have established a fast, safe, accurate and inexpensive procedure for sex typing of canary using DNA extracted from feathers