Bulblet production from node explant grown in vitro in hybrid lilies

Bulblet production from node explant grown in vitro in hybrid lilies

Abstract :An attempt was made to regenerate bulblets from ex vitro node explants of hybrid lilies in the year 2007. Node sections (3-4 mm) isolated from the middle part of the stem in hybrid lilies were cultured on Murashige and Skoog (MS) medium supplemented with several combinations of NAA and BA. Growth regulator-free medium was not effective in inducing bulblet regeneration. A significant increase in the percent of explant producing bulblets and number of bulblets per explant was observed when 2 mg/l NAA was used in combination with 1.5 or 2 mg/l BA. The heaviest bulblets were obtained with 2 mg/l NAA in combination with 1.5 mg/l BA after 90 days of culture. The cultivar Apeldoorn produced greater number of bulblets whereas more weighty bulblets were produced in the cultivar Beartix. 1 or 2 mg/l IBA was most effective in producing roots. The rooted bulblets were hardened with 80-82% survival success after 30 days of transfer in the pots. Keywords: Asiatic hybrids; Bulblet; Growth regulators; Micropropagation; Node explant; Oriental hybrids