The study of hydroalcoholic extract of Ziziphora tenuior on visceral pain with writhing test in mice

Ziziphora tenuior is a traditional herbal medicine widely used as a mild sedative, spasmolytic and antibacterial agent. In this study the effect of hydroalcoholic extract of Ziziphora tenuior on visceral pain was investigated. This experimental study performed on 40 N-MRI male mice (28±3g). Animals were randomly grouped into control, positive control and receivers of Ziziphora tenuior extract. Control and positive control groups received normal saline and indomethacin (5mg/kg), respectively. Treatment groups were injected with 50, 75 and 100 mg/kg of hydroalcoholic extract of Ziziphora tenuior. All injections were performed intraperitonealy (ip). Animals were injected with acetic acid 0.6% (10ml/kg) for visceral pain induction, 30 minutes after of each intraperitoneal administration. Antinociceptive effects were recorded by counting the number of writhes during 30 minutes. The data were analyzed by SPSS statistical software and One-Way ANOVA test. The significant was shown with (p <0.05). Hydroalcoholic extract of Ziziphora tenuior at 50, 75 and 100 mg/kg and Indomethacin (5mg/kg) induced a significant reduction in pain response when compared to control group. Meanwhile, 75mg/kg of extract showed the most effect (p <0.05). This study confirms the antinociceptive properties of Ziziphora tenuior in comparison to Indomethacin, however further studies are necessary to find out a suitable place for antispasmodic effect of Ziziphora tenuior.