The Effect of Hydro-Alcoholic Extract of Mentha-Piperita on the Ileum Movement in Guinea Pig

Background and Much evidence indicates that Mentha-piperita (MP) is traditionally applied for the treatment of gastrointestinal disorders and probably reduces smooth muscle contraction and bowel movement. The present study was conducted to determine the effect of MP Hydroalcoholic extract on ileum movement in guinea pig ileum in invitro model. Methods and Materials: In this experimental study we used 5 male guinea pigs (200-300 gr). After killing the guinea pigs at least 6 pieces of ileum 2-3 cm long were dissected and established in normal Tyrod solution plus carbogen gas in 37ºC. Then each tissue piece was fixed between two electrodes connected to stimulator apparatus. The contraction of ileum was carried to a physiograph by an isotonic transducer and contractions were recorded. Then solutions with 5 concentrations of MP extract were added to the organ bath during ileum stimulation with stimulator. Then the physiograph recorded the contractions again. At least 6-7 responses were recorded. Findings revealed that addition of Hydroalcoholic extract of MP in concentrations of 5 15 50 150 500 mg/ml during 0.1 Hz stimulation decreased contraction duration in a dose-dependent manner. The percent of these changes were 27% 30.8% 41% 61% and 75% respectively. Activity of 50% of this effect of MP was found to be in the dose of 50 mg/ml (p<0/05)....